Lipid Test Cassettes

Contents
Ranges	Storage	Prices	Information Request

The following lipid cassettes are available for use with the L·D·X®:

Total Cholesterol plus HDL panel	Total Cholesterol - HDL plus Glucose panel	Lipid Profile (Total cholesterol, HDL, Triglycerides, calculated LDL & VLDL)	Lipid Profile plus Glucose

The Cholestech L·D·X® system combines enzymatic methodology with solid phase technology to measure total cholesterol, HDL cholesterol, triglycerides and glucose.

Samples for testing can be whole blood from a fingerstick (collected in lithium heparin coated capillary tubes) or venipuncture.

A unique system in the cassette separates the plasma from the blood cells. A portion of the plasma flows to the right side of the cassette and is transferred to the total cholesterol and triglyceride reaction pads; Simultaneously, plasma flows to the left side of the cassette where the low density lipoproteins (LDL and VLDL) are precipitated with dextran sulphate and magnesium acetate precipitating agent. The filtrate, containing both HDL and glucose is transferred to those reaction pads.

Cholesterol esterase hydrolyses the cholesterol esters to free cholesterol and fatty acid; cholesterol oxidase then oxidises free cholesterol; the resulting peroxide reacts with TOOS (4-aminoantipyrine and N-ethyl-N-sulfohydroxypropyl-m-toluidine, sodium salt) in a reaction catalysed by horseradish peroxidase, to form a purple coloured quinoneimine dye - proportional to the TC and HDL cholesterol concentrations in the sample.

In a similar way the L·D·X® measures the glucose concentration by an enzymatic method that uses glucose oxidase to catalyse the oxidation of glucose to peroxide.

Triglycerides concentration is measured via an enzymatic method based on the hydrolysis of triglycerides by cholesterol esterase to glycerol and free fatty acids. Glycerol, in a reaction catalysed by glycerol kinase is converted to glycerol-3-phosphate, which is in turn oxidised to peroxide. The colour reaction is the same as for TC, HDL and glucose.

The brown, magnetic stripe on each cassette contains the calibration information required for the L·D·X® analyser to convert the reflectance reading to the total cholesterol , HDL cholesterol, triglycerides and glucose concentrations.

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Ranges for Lipid Measurement using Cholestech L·D·X®

(As detailed in Medical Devices Agency document MDA 95/23)

Range	TC	HDL	TRIGS	LDL(*calculated)
LOW	2.58	0.39	0.51	1.96
HIGH	12.92	2.59	7.34	6.98
*based on Friedwald equation LDL=TC-((TRIG/2.19)+HDL)

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Storage

All cassettes should be refrigerated at 2-8 °C and not frozen.

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Prices

For information on the cost of these products, please speak to a representative on +44 (0)1793 840073 and quote the following code. Alternatively, complete the online Quotation Request Form.

Code	Description
10-420	Total Cholesterol plus HDL Panel
10-430	TC·HDL·Glucose Panel
10-440	Lipid Profile
10-450	Lipid Profile plus Glucose

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Information Request Form

For further information, please complete the online request form or call on 01793 840073.

Online Request Form